Expression of dopamine D1-receptor mRNA in the carotid body of adult rabbits, cats and rats.

Publication Type:

Journal Article


Neurosci Res, Volume 31, Issue 2, p.147-54 (1998)


Animals, Base Sequence, Blotting, Northern, Carotid Body, Cats, Molecular Sequence Data, Rabbits, Rats, Rats, Sprague-Dawley, Receptors, Dopamine D1, RNA, Messenger, Sequence Homology, Nucleic Acid, Superior Cervical Ganglion


<p>Dopamine is a major neurotransmitter in the carotid body of several animal species and its functional role at the level of peripheral arterial chemoreflex pathway is attributed to the presence of the dopamine D2-receptors. We present evidence that the dopamine D1-receptor mRNA is also expressed in the carotid body of adult rabbits, cats and rats. A DNA fragment of 611 bp of this receptor was first isolated from rabbit. The nucleic acid sequence of this fragment was found to be 84.5% identical to that of rat. This specific 611 bp fragment was used as a probe to detect, either by Northern analysis or by the reverse transcription-polymerase chain reaction, the dopamine D1-receptor mRNA. The results revealed the presence of dopamine D1-receptor transcript in the carotid body as well as in the petrosal ganglion and the superior cervical ganglion from the three animal models studied here. The physiological significance of dopamine D1-receptor expression in the carotid body is discussed.</p>

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