The human decapping scavenger enzyme DcpS modulates microRNA turnover.

Publication Type:

Journal Article

Source:

Sci Rep, Volume 5, p.16688 (2015)

Keywords:

Active Transport, Cell Nucleus, Amino Acid Sequence, Blotting, Northern, Blotting, Western, Cell Nucleus, Endoribonucleases, Fluorescent Antibody Technique, Gene Expression Profiling, HEK293 Cells, Humans, MicroRNAs, Molecular Sequence Data, Mutation, Oligonucleotide Array Sequence Analysis, Reverse Transcriptase Polymerase Chain Reaction, RNA Caps, RNA Interference, Sequence Homology, Amino Acid

Abstract:

<p>The decapping scavenger enzyme DcpS is known for its role in hydrolyzing the cap structure following mRNA degradation. Recently, we discovered a new function in miRNA degradation activation for the ortholog of DcpS in C. elegans. Here we show that human DcpS conserves its role in miRNA turnover. In human cells, DcpS is a nucleocytoplasmic shuttling protein that activates miRNA degradation independently of its scavenger decapping activity in the cytoplasmic compartment. We also demonstrate that this new function for DcpS requires the contribution of the 5'-3' exonuclease Xrn2. Our findings support a conserved role of DcpS as a modulator of miRNA turnover in animals.</p>

Funding / Support / Partners

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