Label-free second-harmonic phase imaging of biological specimen by digital holographic microscopy.

Publication Type:

Journal Article

Authors:

Shaffer, Etienne; Moratal, Corinne; Magistretti, Pierre; Pierre Marquet; Depeursinge, Christian

Source:

Opt Lett, Volume 35, Issue 24, p.4102-4 (2010)

Keywords:

Animals, Dermis, Epidermis, Holography, Image Processing, Computer-Assisted, Mice, Microscopy, Molecular Imaging

Abstract:

<p>We have previously developed a new way for nonscanning second-harmonic generation (SHG) microscopy [Opt. Lett. 34, 2450 (2009)]. Based on digital holography, this technique captures, in single-shot hologram acquisition, both the amplitude and the phase of a coherent SHG radiation, which makes possible second harmonic phase microscopy. In this work, we present holographic SHG phase microscopy of a label-free biological tissue and discuss its added value to SHG microscopy.</p>