Ligand-induced clustering of EGF receptors: a quantitative study by fluorescence image moment analysis.

Publication Type:

Journal Article


Biophys Chem, Volume 161, p.50-3 (2012)


Animals, CHO Cells, Cluster Analysis, Cricetinae, Cricetulus, ErbB Receptors, Green Fluorescent Proteins, Ligands, Microscopy, Fluorescence


<p>Fluorescence microscopy is widely used in the life sciences, but largely for qualitative imaging. Here we apply a bioanalytical technique, fluorescence image moment analysis, to demonstrate how the distribution of the fluorescent molecules can be measured directly from confocal microscopy images. We measured the oligomerization state of EGF-eGPF receptors expressed in CHO-K1 cells in situ.</p>

Funding / Support / Partners

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