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Maintaining polarization in polarimetric multiphoton microscopy.

Publication Type:

Journal Article

Source:

J Biophotonics, Volume 8, Issue 11-12, p.884-8 (2015)

Keywords:

Collagen, Demyelinating Diseases, Equipment Design, Fluorescent Dyes, Lysophosphatidylcholines, Microscopy, Polarization, Models, Theoretical, Myelin Sheath, Oxazines

Abstract:

Polarimetric measurements in multiphoton microscopy can reveal information about the local molecular order of a sample. However, the presence of a dichroic through which the excitation beam propagates will generally scramble its polarization. We propose a simple scheme whereby a second properly-oriented compensation dichroic is used to negate any alteration regardless of the wavelength and the initial polarization. We demonstrate how this robust and rapid approach simplifies polarimetric measurements in second-harmonic generation, two-photon excited fluorescence and coherent anti-Stokes Raman scattering. Illustration of the polarization maintaining strategy with the compensating dichroic oriented such that its s- and p-axes are interchanged with these of the primary dichroic.

Funding / Support / Partners

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