@article{pmid38545127,

title = {Understanding the nervous system: lessons from Frontiers in Neurophotonics},

author = {Yves De Koninck and Johanna Alonso and Stéphane Bancelin and Jean-Claude Béïque and Erik Bélanger and Catherine Bouchard and Marco Canossa and Johan Chaniot and Daniel Choquet and Marie-Ève Crochetière and Nanke Cui and Lydia Danglot and Paul De Koninck and Anna Devor and Mathieu Ducros and Angela M Getz and Mohamed Haouat and Iván Coto Hernández and Nate Jowett and Iason Keramidis and Céline Larivière-Loiselle and Flavie Lavoie-Cardinal and Harold D MacGillavry and Asiye Malkoç and Mattia Mancinelli and Pierre Marquet and Steven Minderler and Maxime Moreaud and U Valentin Nägerl and Katerina Papanikolopoulou and Marie-Eve Paquet and Lorenzo Pavesi and David Perrais and Romain Sansonetti and Martin Thunemann and Beatrice Vignoli and Jenny Yau and Clara Zaccaria},

doi = {10.1117/1.NPh.11.1.014415},

issn = {2329-423X},

year  = {2024},

date = {2024-01-01},

journal = {Neurophotonics},

volume = {11},

number = {1},

pages = {014415},

abstract = {The Frontiers in Neurophotonics Symposium is a biennial event that brings together neurobiologists and physicists/engineers who share interest in the development of leading-edge photonics-based approaches to understand and manipulate the nervous system, from its individual molecular components to complex networks in the intact brain. In this Community paper, we highlight several topics that have been featured at the symposium that took place in October 2022 in Québec City, Canada.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid38550388,

title = {Special Section Guest Editorial: Frontiers in Neurophotonics},

author = {Yves De Koninck and Paul De Koninck and Anna Devor and Flavie Lavoie-Cardinal},

doi = {10.1117/1.NPh.11.1.014401},

issn = {2329-423X},

year  = {2024},

date = {2024-01-01},

journal = {Neurophotonics},

volume = {11},

number = {1},

pages = {014401},

abstract = {The editorial presents the two-part Special Section on Frontiers in Neurophotonics.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid37551444,

title = {Restoring neuronal chloride extrusion reverses cognitive decline linked to Alzheimer's disease mutations},

author = {Iason Keramidis and Brendan B McAllister and Julien Bourbonnais and Feng Wang and Dominique Isabel and Edris Rezaei and Romain Sansonetti and Phil Degagne and Justin P Hamel and Mojtaba Nazari and Samsoon Inayat and Jordan C Dudley and Annie Barbeau and Lionel Froux and Marie-Eve Paquet and Antoine G Godin and Majid H Mohajerani and Yves De Koninck},

doi = {10.1093/brain/awad250},

issn = {1460-2156},

year  = {2023},

date = {2023-12-01},

journal = {Brain},

volume = {146},

number = {12},

pages = {4903--4915},

abstract = {Disinhibition during early stages of Alzheimer's disease is postulated to cause network dysfunction and hyperexcitability leading to cognitive deficits. However, the underlying molecular mechanism remains unknown. Here we show that, in mouse lines carrying Alzheimer's disease-related mutations, a loss of neuronal membrane potassium-chloride cotransporter KCC2, responsible for maintaining the robustness of GABAA-mediated inhibition, occurs pre-symptomatically in the hippocampus and prefrontal cortex. KCC2 downregulation was inversely correlated with the age-dependent increase in amyloid-β 42 (Aβ42). Acute administration of Aβ42 caused a downregulation of membrane KCC2. Loss of KCC2 resulted in impaired chloride homeostasis. Preventing the decrease in KCC2 using long term treatment with CLP290 protected against deterioration of learning and cortical hyperactivity. In addition, restoring KCC2, using short term CLP290 treatment, following the transporter reduction effectively reversed spatial memory deficits and social dysfunction, linking chloride dysregulation with Alzheimer's disease-related cognitive decline. These results reveal KCC2 hypofunction as a viable target for treatment of Alzheimer's disease-related cognitive decline; they confirm target engagement, where the therapeutic intervention takes place, and its effectiveness.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid37792939,

title = {Enhanced harm detection following maternal separation: Transgenerational transmission and reversibility by inhaled amiloride},

author = {Marco Battaglia and Orlane Rossignol and Louis-Etienne Lorenzo and Jasmin Deguire and Antoine G Godin and Francesca R D'Amato and Yves De Koninck},

doi = {10.1126/sciadv.adi8750},

issn = {2375-2548},

year  = {2023},

date = {2023-10-01},

journal = {Sci Adv},

volume = {9},

number = {40},

pages = {eadi8750},

abstract = {Early-life adversities are associated with altered defensive responses. Here, we demonstrate that the repeated cross-fostering (RCF) paradigm of early maternal separation is associated with enhancements of distinct homeostatic reactions: hyperventilation in response to hypercapnia and nociceptive sensitivity, among the first generation of RCF-exposed animals, as well as among two successive generations of their normally reared offspring, through matrilineal transmission. Parallel enhancements of acid-sensing ion channel 1 (ASIC1), ASIC2, and ASIC3 messenger RNA transcripts were detected transgenerationally in central neurons, in the medulla oblongata, and in periaqueductal gray matter of RCF-lineage animals. A single, nebulized dose of the ASIC-antagonist amiloride renormalized respiratory and nociceptive responsiveness across the entire RCF lineage. These findings reveal how, following an early-life adversity, a biological memory reducible to a molecular sensor unfolds, shaping adaptation mechanisms over three generations. Our findings are entwined with multiple correlates of human anxiety and pain conditions and suggest nebulized amiloride as a therapeutic avenue.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid37362387,

title = {Millimetric scale two-photon Bessel-Gauss beam light sheet microscopy with three-axis isotropic resolution using an axicon lens},

author = {Cléophace Akitegetse and Thomas Charland and Mireille Quémener and Charles Gora and Véronique Rioux and Michel Piché and Yves De Koninck and Martin Lévesque and Daniel C Côté},

doi = {10.1117/1.NPh.10.3.035002},

issn = {2329-423X},

year  = {2023},

date = {2023-07-01},

journal = {Neurophotonics},

volume = {10},

number = {3},

pages = {035002},

abstract = {SIGNIFICANCE: Typical light sheet microscopes suffer from artifacts related to the geometry of the light sheet. One main inconvenience is the non-uniform thickness of the light sheet obtained with a Gaussian laser beam.nnAIM: We developed a two-photon light sheet microscope that takes advantage of a thin and long Bessel-Gauss beam illumination to increase the sheet extent without compromising the resolution.nnAPPROACH: We use an axicon lens placed directly at the output of an amplified femtosecond laser to produce a long Bessel-Gauss beam on the sample. We studied the dopaminergic system and its projections in a whole cleared mouse brain.nnRESULTS: Our light sheet microscope allows an isotropic resolution of  in all three axes of the scanned volume while keeping a millimetric-sized field of view, and a fast acquisition rate of up to . With slight modifications to the optical setup, the sheet extent can be increased to 6 mm.nnCONCLUSION: The proposed system's sheet extent and resolution surpass currently available systems, enabling the fast imaging of large specimens.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid37475396,

title = {Improving flat fluorescence microscopy in scattering tissue through deep learning strategies},

author = {Mohamad Feshki and Sylvain Martel and Yves De Koninck and Benoit Gosselin},

doi = {10.1364/OE.489677},

issn = {1094-4087},

year  = {2023},

date = {2023-07-01},

journal = {Opt Express},

volume = {31},

number = {14},

pages = {23008--23026},

abstract = {Intravital microscopy in small animals growingly contributes to the visualization of short- and long-term mammalian biological processes. Miniaturized fluorescence microscopy has revolutionized the observation of live animals' neural circuits. The technology's ability to further miniaturize to improve freely moving experimental settings is limited by its standard lens-based layout. Typical miniature microscope designs contain a stack of heavy and bulky optical components adjusted at relatively long distances. Computational lensless microscopy can overcome this limitation by replacing the lenses with a simple thin mask. Among other critical applications, Flat Fluorescence Microscope (FFM) holds promise to allow for real-time brain circuits imaging in freely moving animals, but recent research reports show that the quality needs to be improved, compared with imaging in clear tissue, for instance. Although promising results were reported with mask-based fluorescence microscopes in clear tissues, the impact of light scattering in biological tissue remains a major challenge. The outstanding performance of deep learning (DL) networks in computational flat cameras and imaging through scattering media studies motivates the development of deep learning models for FFMs. Our holistic ray-tracing and Monte Carlo FFM computational model assisted us in evaluating deep scattering medium imaging with DL techniques. We demonstrate that physics-based DL models combined with the classical reconstruction technique of the alternating direction method of multipliers (ADMM) perform a fast and robust image reconstruction, particularly in the scattering medium. The structural similarity indexes of the reconstructed images in scattering media recordings were increased by up to 20% compared with the prevalent iterative models. We also introduce and discuss the challenges of DL approaches for FFMs under physics-informed supervised and unsupervised learning.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid37171242,

title = {We need to talk: The urgent conversation on chronic pain, mental health, prescribing patterns and the opioid crisis},

author = {Marco Battaglia and Cornelius B Groenewald and Fiona Campbell and Simona Scaini and Yves De Koninck and Jennifer Stinson and Patrick D Quinn},

doi = {10.1177/02698811221144635},

issn = {1461-7285},

year  = {2023},

date = {2023-05-01},

journal = {J Psychopharmacol},

volume = {37},

number = {5},

pages = {437--448},

abstract = {The opioid crisis' pathways from first exposure onwards to eventual illnesses and fatalities are multiple, intertwined and difficult to dissect. Here, we offer a multidisciplinary appraisal of the relationships among mental health, chronic pain, prescribing patterns worldwide and the opioid crisis. Because the opioid crisis' toll is especially harsh on young people, emphasis is given on data regarding the younger strata of the population. Because analgesic opioid prescription constitute a recognised entry point towards misuse, opioid use disorder, and ultimately overdose, prescribing patterns across different countries are examined as a modifiable hazard factor along these pathways of risk. Psychiatrists are called to play a more compelling role in this urgent conversation, as they are uniquely placed to provide synthesis and lead action among the different fields of knowledge and care that lie at the crossroads of the opioid crisis. Psychiatrists are also ideally positioned to gauge and disseminate the foundations for diagnosis and clinical management of mental conditions associated with chronic pain, including the identification of hazardous and protective factors. It is our hope to spark more interdisciplinary exchanges and encourage psychiatrists worldwide to become leaders in an urgent conversation with interlocutors from the clinical and basic sciences, policy makers and stakeholders including clients and their families.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid34363210,

title = {Remote local photoactivation of morphine produces analgesia without opioid-related adverse effects},

author = {Marc López-Cano and Joan Font and Ester Aso and Kristoffer Sahlholm and Gisela Cabré and Jesús Giraldo and Yves De Koninck and Jordi Hernando and Amadeu Llebaria and Víctor Fernández-Dueñas and Francisco Ciruela},

doi = {10.1111/bph.15645},

issn = {1476-5381},

year  = {2023},

date = {2023-04-01},

journal = {Br J Pharmacol},

volume = {180},

number = {7},

pages = {958--974},

abstract = {BACKGROUND AND PURPOSE: Opioid-based drugs are the gold standard medicines for pain relief. However, tolerance and several side effects (i.e. constipation and dependence) may occur upon chronic opioid administration. Photopharmacology is a promising approach to improve the benefit/risk profiles of these drugs. Thus, opioids can be locally activated with high spatiotemporal resolution, potentially minimizing systemic-mediated adverse effects. Here, we aimed at developing a morphine photo-derivative (photocaged morphine), which can be activated upon light irradiation both in vitro and in vivo.nnEXPERIMENTAL APPROACH: Light-dependent activity of pc-morphine was assessed in cell-based assays (intracellular calcium accumulation and electrophysiology) and in mice (formalin animal model of pain). In addition, tolerance, constipation and dependence were investigated in vivo using experimental paradigms.nnKEY RESULTS: In mice, pc-morphine was able to elicit antinociceptive effects, both using external light-irradiation (hind paw) and spinal cord implanted fibre-optics. In addition, remote morphine photoactivation was devoid of common systemic opioid-related undesired effects, namely, constipation, tolerance to the analgesic effects, rewarding effects and naloxone-induced withdrawal.nnCONCLUSION AND IMPLICATIONS: Light-dependent opioid-based drugs may allow effective analgesia without the occurrence of tolerance or the associated and severe opioid-related undesired effects.nnLINKED ARTICLES: This article is part of a themed issue on Advances in Opioid Pharmacology at the Time of the Opioid Epidemic. To view the other articles in this section visit http://onlinelibrary.wiley.com/doi/10.1111/bph.v180.7/issuetoc.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid34847973,

title = {An Indigenous Lens on Priorities for the Canadian Brain Research Strategy},

author = {Melissa L Perreault and Malcolm King and Chelsea Gabel and Christopher J Mushquash and Yves De Koninck and Ashley Lawson and Caterina Marra and Caroline Ménard and Jennie Z Young and Judy Illes},

doi = {10.1017/cjn.2021.501},

issn = {0317-1671},

year  = {2023},

date = {2023-01-01},

journal = {Can J Neurol Sci},

volume = {50},

number = {1},

pages = {96--98},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid36656715,

title = {Time-dependent and selective microglia-mediated removal of spinal synapses in neuropathic pain},

author = {Noosha Yousefpour and Samantha Locke and Haley Deamond and Chengyang Wang and Lucas Marques and Manon St-Louis and Johanne Ouellette and Arkady Khoutorsky and Yves De Koninck and Alfredo Ribeiro-da-Silva},

doi = {10.1016/j.celrep.2023.112010},

issn = {2211-1247},

year  = {2023},

date = {2023-01-01},

journal = {Cell Rep},

volume = {42},

number = {1},

pages = {112010},

abstract = {Neuropathic pain is a debilitating condition resulting from damage to the nervous system. Imbalance of spinal excitation and inhibition has been proposed to contribute to neuropathic pain. However, the structural basis of this imbalance remains unknown. Using a preclinical model of neuropathic pain, we show that microglia selectively engulf spinal synapses that are formed by central neurons and spare those of peripheral sensory neurons. Furthermore, we reveal that removal of inhibitory and excitatory synapses exhibits distinct temporal patterns, in which microglia-mediated inhibitory synapse removal precedes excitatory synapse removal. We also find selective and gradual increase in complement depositions on dorsal horn synapses that corresponds to the temporal pattern of microglial synapse pruning activity and type-specific synapse loss. Together, these results define a specific role for microglia in the progression of neuropathic pain pathogenesis and implicate these immune cells in structural remodeling of dorsal horn circuitry.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}