The editor of the scientific journal Scientific Reports has published a list of key articles in the field of super-resolution microscopy, including a publication by Paul De Koninck, Flavie Lavoie-Cardinal and their team at the CERVO research center.
Super-resolution microscopy encompasses optical microscopy techniques that produce images in which structures are laterally resolved beyond the diffraction limit of light (250nm). These techniques have revolutionized fluorescence microscopy and enabled probing cellular structure in unprecedented detail. Since Eric Betzig, Stefan Hell and William Moerner were awarded the 2014 Nobel prize in Chemistry for developing super resolved fluorescence microscopy, the methodologies have continuously evolved and are becoming increasingly amenable to real time and quantitative imaging. Super resolution imaging is now also being used in conjunction with other microscopy techniques to further extend their abilities to uncover nanoscale detail. This Collection showcases the latest research presenting applications of super resolution microscopy—to characterize cellular ultrastructure and dynamics, in medicine—as well as technological improvements and quantitative methods applied to the techniques.
Read the article by the CERVO teams here:
Lavoie-Cardinal, F., Bilodeau, A., Lemieux, M. et al. Neuronal activity remodels the F-actin based submembrane lattice in dendrites but not axons of hippocampal neurons. Sci Rep 10, 11960 (2020). https://doi.org/10.1038/s41598-020-68180-2