Publication Type:Journal Article
Source:J Biophotonics, Volume 3, Issue 7, p.432-6 (2010)
Keywords:Aniline Compounds, Animals, Calcium, Cell Death, Cell Size, Cells, Cultured, Cerebral Cortex, Fluorescence, Glutamic Acid, Holography, Homeostasis, Intracellular Space, Ions, Mice, Microscopy, Fluorescence, Neurons, Time Factors, Xanthenes
The authors have developed a live-cell multimodality microscope combining epifluorescence with digital holographic microscopy; it has been implemented with a decoupling procedure allowing to separately measure from the quantitative phase important cell parameters including absolute volume, shape and integral intracellular refractive index. In combination with the numerous different specific fluorescent cellular probes, this multimodality microscopy can address important issues in cell biology. This is demonstrated by the study of intracellular calcium homeostasis associated with the change in cell volume, which play a critical role in the excitotoxicity-induced neuronal death.