Enkephalin-immunoreactive nociceptive neurons in the cat spinal cord.

Publication Type:

Journal Article


Neuroreport, Volume 3, Issue 1, p.25-8 (1992)


Animals, Antibodies, Monoclonal, Autoradiography, Cats, Dendrites, Electric Stimulation, Enkephalins, Horseradish Peroxidase, Male, Neurons, Nociceptors, Physical Stimulation, Spinal Cord, Substance P


<p>In this combined electrophysiological-ultrastructural study in the cat spinal cord, we detected enkephalin-like immunoreactivity using internally radio-labelled monoclonal antibodies in functionally characterized neurons which had been filled intracellularly with horseradish peroxidase. Of the 4 neurons included in this study, two were positive for enkephalin immunoreactivity; one was a nociceptive specific neuron in lamina I, the other a wide dynamic range neuron in lamina V. The other two cells were devoid of immunoreactivity for enkephalin; one was a wide dynamic range neuron and the other was a non-nociceptive neuron. These results thus provide a morphological substrate within the spinal dorsal horn for the release of an endogenous opioid following administration of substance P or noxious cutaneous stimulation.</p>

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