CIUSSS Capitale nationale logo

Expression of Human Neurofilament-light Transgene in Mouse Neurons Transplanted into the Brain of Adult Rats.

Publication Type:

Journal Article

Source:

Eur J Neurosci, Volume 3, Issue 8, p.758-763 (1991)

Abstract:

To investigate the expression of nerve cell-specific transgene products in neural transplants, we implanted into the hippocampus of immunosuppressed adult Sprague - Dawley rats cell suspensions obtained from the septal region of the fetal brain of mice that carry the human neurofilament-light (hNF-L) gene. In grafts examined between 3 weeks and 7 months after transplantation, axons and nerve cell somata immunoreacted to antibodies specific to the human NF-L subunit. Thus, the hNF-L protein appears to be a suitable marker of these grafted neurons. Transgenic mice bearing the hNF-L gene may be a convenient source of donor tissue or be used as hosts for neural transplantation studies. Furthermore, the hNF-L promoter/enhancer elements in this transgene may help direct neuronal expression of heterologous genes that could influence nerve cell responses in either the transplant or host tissues.

Financement / Soutien / Partenaires

logo FRQ-S logo ctrn logo fci logo cihr irsc logo nserc logo MESISentinelle nord