A genetically encoded fluorescent biosensor for extracellular L-lactate.

Publication Type:

Journal Article


Nat Commun, Volume 12, Issue 1, p.7058 (2021)


Bacterial Proteins, Binding Sites, Biosensing Techniques, Cell Line, Tumor, Crystallography, X-Ray, Fluorescence, Green Fluorescent Proteins, HEK293 Cells, HeLa Cells, Humans, Lactic Acid, Microscopy, Fluorescence, Periplasmic Proteins, Protein Binding, Recombinant Fusion Proteins, Reproducibility of Results


<p>L-Lactate, traditionally considered a metabolic waste product, is increasingly recognized as an important intercellular energy currency in mammals. To enable investigations of the emerging roles of intercellular shuttling of L-lactate, we now report an intensiometric green fluorescent genetically encoded biosensor for extracellular L-lactate. This biosensor, designated eLACCO1.1, enables cellular resolution imaging of extracellular L-lactate in cultured mammalian cells and brain tissue.</p>

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