A genetically encoded fluorescent biosensor for extracellular L-lactate.

Publication Type:

Journal Article

Authors:

Nasu, Yusuke; Murphy-Royal, Ciaran; Wen, Yurong; Haidey, Jordan N; Molina, Rosana S; Aggarwal, Abhi; Zhang, Shuce; Kamijo, Yuki; Paquet, Marie-Eve; Podgorski, Kaspar; Drobizhev, Mikhail; Bains, Jaideep S; Lemieux, M Joanne; Gordon, Grant R; Campbell, Robert E

Source:

Nat Commun, Volume 12, Issue 1, p.7058 (2021)

Keywords:

Bacterial Proteins, Binding Sites, Biosensing Techniques, Cell Line, Tumor, Crystallography, X-Ray, Fluorescence, Green Fluorescent Proteins, HEK293 Cells, HeLa Cells, Humans, Lactic Acid, Microscopy, Fluorescence, Periplasmic Proteins, Protein Binding, Recombinant Fusion Proteins, Reproducibility of Results

Abstract:

<p>L-Lactate, traditionally considered a metabolic waste product, is increasingly recognized as an important intercellular energy currency in mammals. To enable investigations of the emerging roles of intercellular shuttling of L-lactate, we now report an intensiometric green fluorescent genetically encoded biosensor for extracellular L-lactate. This biosensor, designated eLACCO1.1, enables cellular resolution imaging of extracellular L-lactate in cultured mammalian cells and brain tissue.</p>