Department of Chemical Engineering
Institute for Medical Engineering and Science (IMES)
Picower Institute for Learning and Memory
Massachusetts Institute of Technology
Holistic measurement of diverse functional, anatomical, and molecular traits that span multiple levels, from molecules to cells to an entire system, remains a major challenge in biology. In this talk, I will introduce a series of technologies including CLARITY (Nature, 2013), SWITCH (Cell, 2015), MAP (Nature Biotechnology, 2016), stochastic electrotransport (PNAS, 2015), and SHIELD (Nature Biotechnology, 2018) that enable integrated multiscale imaging and molecular phenotyping of both animal tissues and human clinical samples.
I will discuss how we engineer(1) the physicochemical properties of brain tissues, (2) molecular interactions, and (3) molecular transport all together to achieve integrated brain-wide molecular phenotyping at unprecedented speed and resolution.I will also discuss how these tools can be deployed synergistically to study a broad range of biological questions. We hope that these new technologies will accelerate the pace of discoveryin biomedical research.