Ligand-induced clustering of EGF receptors: a quantitative study by fluorescence image moment analysis.

Publication Type:

Journal Article

Authors:

Sergeev, Mikhail; Swift, Jody L; Antoine G Godin; Wiseman, Paul W

Source:

Biophys Chem, Volume 161, p.50-3 (2012)

Keywords:

Animals, CHO Cells, Cluster Analysis, Cricetinae, Cricetulus, ErbB Receptors, Green Fluorescent Proteins, Ligands, Microscopy, Fluorescence

Abstract:

<p>Fluorescence microscopy is widely used in the life sciences, but largely for qualitative imaging. Here we apply a bioanalytical technique, fluorescence image moment analysis, to demonstrate how the distribution of the fluorescent molecules can be measured directly from confocal microscopy images. We measured the oligomerization state of EGF-eGPF receptors expressed in CHO-K1 cells in situ.</p>