Recognition molecule associated carbohydrate inhibits postsynaptic GABA(B) receptors: a mechanism for homeostatic regulation of GABA release in perisomatic synapses.

Publication Type:

Journal Article

Authors:

Armen Saghatelyan; Snapyan, Marina; Gorissen, Silke; Meigel, Inge; Mosbacher, Johannes; Kaupmann, Klemens; Bettler, Bernhard; Kornilov, Andrei V; Nifantiev, Nikolay E; Sakanyan, Vehary; Schachner, Melitta; Dityatev, Alexander

Source:

Mol Cell Neurosci, Volume 24, Issue 2, p.271-82 (2003)

Keywords:

Animals, Antigens, CD57, Cell Line, gamma-Aminobutyric Acid, Hippocampus, Homeostasis, Humans, Mice, Mice, Inbred C57BL, Rats, Receptors, GABA-B, Synapses

Abstract:

<p>Extracellular matrix molecules are important cues in the shaping of nervous system structure and function. Here, we describe a novel mechanism by which the HNK-1 carbohydrate carried by recognition molecules regulates perisomatic inhibition in the hippocampus. Neutralization of HNK-1 activity by an HNK-1 antibody results in GABA(B) receptor-mediated activation of K(+) currents in CA1 pyramidal cells, which elevates extracellular K(+) concentration and reduces evoked GABA release in perisomatic inhibitory synapses. This mechanism is supported by pharmacological analysis in hippocampal slices and data showing that the HNK-1 carbohydrate binds to GABA(B) receptors and inhibits GABA(B) receptor-activated K(+) currents in a heterologous expression system. We suggest that the HNK-1 carbohydrate is involved in homeostatic regulation of GABA(A) receptor-mediated perisomatic inhibition by suppression of postsynaptic GABA(B) receptor activity.</p>