Resolution and contrast enhancement in coherent anti-Stokes Raman-scattering microscopy.

Publication Type:

Journal Article

Authors:

Gasecka, Alicja; Daradich, Amy; Dehez, Harold; Piché, Michel; Daniel C Côté

Source:

Opt Lett, Volume 38, Issue 21, p.4510-3 (2013)

Keywords:

Animals, Brain Stem, Lasers, Mice, Microscopy, Myelin Sheath, Optical Processes, Spectrum Analysis, Raman

Abstract:

<p>We implement switching laser mode coherent anti-Stokes Raman-scattering (SLAM-CARS) microscopy to enhance the spatial resolution and contrast in label-free vibrational microscopy. The method, based on the intensity difference between two images obtained with Gaussian and doughnut-shaped modes, does not depend on the specimen and relies on minimal modifications of the typical CARS setup. We demonstrate subdiffraction resolution imaging of myelin sheaths in a mouse brainstem. A lateral resolution of 0.36λ(p) is achieved.</p>