Publication Type:Journal Article
Source:Opt Lett, Volume 34, Issue 5, p.653-5 (2009)
Keywords:Cells, Equipment Design, Erythrocyte Membrane, Holography, Humans, Microscopy, Tomography, Optical
We present first results on a method enabling mechanical scanning-free tomography with submicrometer axial resolution by multiple-wavelength digital holographic microscopy. By sequentially acquiring reflection holograms and summing 20 wavefronts equally spaced in spatial frequency in the 485-670 nm range, we are able to achieve a slice-by-slice tomographic reconstruction with a 0.6-1 microm axial resolution in a biological medium. The method is applied to erythrocytes investigation to retrieve the cellular membrane profile in three dimensions.