Super-resolution microscopy approaches for live cell imaging.

Publication Type:

Journal Article

Authors:

Antoine G Godin; Lounis, Brahim; Cognet, Laurent

Source:

Biophys J, Volume 107, Issue 8, p.1777-1784 (2014)

Keywords:

Imaging, Three-Dimensional, Limit of Detection, Microscopy, Fluorescence, Single-Cell Analysis

Abstract:

<p>By delivering optical images with spatial resolutions below the diffraction limit, several super-resolution fluorescence microscopy techniques opened new opportunities to study biological structures with details approaching molecular structure sizes. They have now become methods of choice for imaging proteins and their nanoscale dynamic organizations in live cells. In this mini-review, we describe and compare the main far-field super-resolution approaches that allow studying endogenous or overexpressed proteins in live cells.</p>