In vivo confocal and multiphoton microendoscopy.

Publication Type:

Journal Article

Authors:

Kim, Pilhan; Puoris'haag, Mehron; Daniel C Côté; Lin, Charles P; Yun, Seok H

Source:

J Biomed Opt, Volume 13, Issue 1, p.010501 (2008)

Keywords:

Endoscopes, Equipment Design, Equipment Failure Analysis, Microscopy, Confocal, Microscopy, Fluorescence, Multiphoton, Miniaturization, Reproducibility of Results, Sensitivity and Specificity

Abstract:

<p>The ability to conduct high-resolution fluorescence imaging in internal organs of small animal models in situ and over time can make a significant impact in biomedical research. Toward this goal, we developed a real-time confocal and multiphoton endoscopic imaging system. Using 1-mm-diameter endoscopes based on gradient index lenses, we demonstrate video-rate multicolor multimodal imaging with cellular resolution in live mice.</p>