@article{pmid39706384,

title = {Task-specific temporal prediction mechanisms revealed by motor and electroencephalographic indicators},

author = {Alana Arrouet and José Eduardo Marques-Carneiro and Pierre Marquet and Anne Giersch},

doi = {10.1016/j.neuroimage.2024.120982},

issn = {1095-9572},

year  = {2025},

date = {2025-02-01},

journal = {Neuroimage},

volume = {306},

pages = {120982},

abstract = {Time prediction is pervasive, and it is unclear whether it is supra-modal or task-specific. This study aimed to investigate the role of motor temporal prediction in preparing to stop a movement following a sensory stimulus. Participants performed a straight-line movement with their finger until a target signal, which occurred after a short or long foreperiod. In one task, participants changed movement direction between trials (multidirectional task), while in the other, they always moved in the same direction (unidirectional task). The motor trajectory and EEG signals were continuously recorded. During the foreperiod, participants slowed down their movement, reflecting preparation to stop. To assess the influence of motor temporal prediction we examined how a given trial influences performance on the subsequent trial (sequential effect) when the movement changes or stays the same (multi- vs. unidirectional). In the unidirectional task, but not in the multidirectional task we found sequential effects on several behavioural parameters. In contrast, sequential effects were observed in both tasks on EEG results. This study revealed a temporal prediction related to motor movement (behavioural indicators), and a temporal preparation while waiting for the target (EEG indicator). These findings highlight the importance of considering various temporal prediction mechanisms.},

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pubstate = {published},

tppubtype = {article}

}

@article{pmid39625537,

title = {Mathematical properties of pump-leak-cotransport models},

author = {Vincent Ouellet and Nicolas Doyon and Antoine G Godin and Pierre Marquet},

doi = {10.1007/s00285-024-02163-z},

issn = {1432-1416},

year  = {2024},

date = {2024-12-01},

journal = {J Math Biol},

volume = {90},

number = {1},

pages = {2},

abstract = {Models of ordinary differential equations are often used to describe the electrical, ionic and volumetric responses of cells to external stimuli. Although these cellular models are often solved numerically, rigorous evidence regarding their steady state solutions is scarce. In this work, we provide a formalism defining the conditions ensuring the existence and uniqueness of a steady-state solution in a large class of models including leak channels, a pump and cotransporters. Our work generalizes previous results and provides explicit conditions that a model must satisfy to guarantee the existence and uniqueness of a steady state.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid39474363,

title = {Quantitative estimation of optical properties in bilayer media within the subdiffusive regime using a tilted fiber-optic probe in diffuse reflectance spectroscopy, part 1: a theoretical framework for designing probe geometry},

author = {Philippe De Tillieux and Maxime Baillot and Pierre Marquet},

doi = {10.1117/1.JBO.29.10.105001},

issn = {1560-2281},

year  = {2024},

date = {2024-10-01},

journal = {J Biomed Opt},

volume = {29},

number = {10},

pages = {105001},

abstract = {SIGNIFICANCE: As biological tissues are highly heterogeneous, there is a great interest in developing non-invasive optical approaches capable of characterizing them in a very localized manner. Obtaining accurate absolute values of the local optical properties from the measured reflectance requires finding a probe geometry, which allows us to solve this inverse problem robustly and reliably despite neglecting the higher-order moments of the scattering phase function.nnAIM: Our goal is to develop a theoretical framework for designing tilted-fiber diffuse reflectance probes that allow quantitative estimation of the optical properties corresponding to limited tissue volume (typically a few cubic millimeters).nnAPPROACH: Relationships among probe geometry, sampled tissue volume, and robustness of the inverse solver to calculate optical properties from reflectance are studied using Monte Carlo simulations.nnRESULTS: The analysis of the number of scattering events of the collected photons leads to the establishment of relationships among the probe geometry, the sampled tissue volume, and the validity of a subdiffusive regime for the reflectance.nnCONCLUSIONS: A methodology is proposed for the design of new compact probes with tilted fiber geometry that can quantitatively estimate the values of the optical coefficients in a localized manner within living biological tissues by recording diffuse reflectance spectra.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid39474361,

title = {Quantitative estimation of optical properties in bilayer media within the subdiffusive regime using tilted fiber-optic probe diffuse reflectance spectroscopy, part 2: probe design, realization, and experimental validation},

author = {Philippe De Tillieux and Maxime Baillot and Pierre Marquet},

doi = {10.1117/1.JBO.29.10.105002},

issn = {1560-2281},

year  = {2024},

date = {2024-10-01},

journal = {J Biomed Opt},

volume = {29},

number = {10},

pages = {105002},

abstract = {SIGNIFICANCE: Tissues like skin have a layered structure where each layer's optical properties vary significantly. However, traditional diffuse reflectance spectroscopy assumes a homogeneous medium, often leading to estimations that reflects the properties of neither layer. There's a clear need for probes that can precisely measure the optical properties of layered tissues.nnAIM: This paper aims to design a diffuse reflectance probe capable of accurately estimating the optical properties of bilayer tissues in the subdiffusive regime.nnAPPROACH: Using Monte Carlo simulations, we evaluated key geometric factors-fiber placement, tilt angle, diameter, and numerical aperture-on optical property estimation, following the methodology in Part I. A robust design is proposed that balances accurate intrinsic optical property (IOP) calculations with practical experimental constraints.nnRESULTS: The designed probe, featuring eight illumination and eight detection fibers with varying spacings and tilt angles. The estimation error of the IOP calculation for bilayer phantoms is less than 20% for top layers with thicknesses between 0.2 and 1.0 mm.nnCONCLUSION: Building on the approach from Part I and using a precise calibration, the probe effectively quantified and distinguished the IOPs of bilayer samples, particularly those relevant to early skin pathology detection and characterization.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid38545127,

title = {Understanding the nervous system: lessons from Frontiers in Neurophotonics},

author = {Yves De Koninck and Johanna Alonso and Stéphane Bancelin and Jean-Claude Béïque and Erik Bélanger and Catherine Bouchard and Marco Canossa and Johan Chaniot and Daniel Choquet and Marie-Ève Crochetière and Nanke Cui and Lydia Danglot and Paul De Koninck and Anna Devor and Mathieu Ducros and Angela M Getz and Mohamed Haouat and Iván Coto Hernández and Nate Jowett and Iason Keramidis and Céline Larivière-Loiselle and Flavie Lavoie-Cardinal and Harold D MacGillavry and Asiye Malkoç and Mattia Mancinelli and Pierre Marquet and Steven Minderler and Maxime Moreaud and U Valentin Nägerl and Katerina Papanikolopoulou and Marie-Eve Paquet and Lorenzo Pavesi and David Perrais and Romain Sansonetti and Martin Thunemann and Beatrice Vignoli and Jenny Yau and Clara Zaccaria},

doi = {10.1117/1.NPh.11.1.014415},

issn = {2329-423X},

year  = {2024},

date = {2024-01-01},

journal = {Neurophotonics},

volume = {11},

number = {1},

pages = {014415},

abstract = {The Frontiers in Neurophotonics Symposium is a biennial event that brings together neurobiologists and physicists/engineers who share interest in the development of leading-edge photonics-based approaches to understand and manipulate the nervous system, from its individual molecular components to complex networks in the intact brain. In this Community paper, we highlight several topics that have been featured at the symposium that took place in October 2022 in Québec City, Canada.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid38007982,

title = {Risk factors for mood disorders among offspring of parents with bipolar disorder: Findings from a discordant-sibling study},

author = {Francesca Di Giacomo and Marie-Pierre F Strippoli and Enrique Castelao and Joëlle Rosselet Amoussou and Mehdi Gholam and Setareh Ranjbar and Jennifer Glaus and Pierre Marquet and Martin Preisig and Kerstin Jessica Plessen and Caroline L Vandeleur},

doi = {10.1016/j.psychres.2023.115615},

issn = {1872-7123},

year  = {2023},

date = {2023-12-01},

journal = {Psychiatry Res},

volume = {330},

pages = {115615},

abstract = {The purpose of this naturalistic, prospective study was to identify risk factors for mood disorders in offspring of parents with bipolar disorder (BPD) using the discordant-sibling design by comparing premorbid psychopathology or symptoms, temperament, personality traits and coping style as well as the perception of family-related characteristics among affected and unaffected siblings within the same family. This approach controls for confounding by unmeasured genetic and environmental factors shared within families. Our sample comprised 24 families of a parent with BPD with at least one child that developed BPD or major depressive disorder (n = 31), and at least one child who did not. Offspring were followed for a mean duration of 16.2 (s.d: 4.6) years. Information was collected from the offspring themselves. Generalized linear mixed models only revealed differences in three dimensions of the Dimension of Temperament Survey-Revised (DOTS-R) version: Offspring with mood disorders scored higher on "Approach-withdrawal", "Rhythmicity for daily habits", and "Task orientation" than their unaffected siblings. The higher scores, and not lower scores as expected, on these temperament dimensions observed in offspring that subsequently developed mood disorders may reflect increased vulnerability, but they could also mirror premorbid mood swings or strategies to cope with them.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid37335579,

title = {Automated Dual-Mode Cell Monitoring To Simultaneously Explore Calcium Dynamics and Contraction-Relaxation Kinetics within Drug-Treated Stem Cell-Derived Cardiomyocytes},

author = {Keyvan Jaferzadeh and Benjamin Rappaz and Youhyun Kim and Bo-Kyoung Kim and Inkyu Moon and Pierre Marquet and Gerardo Turcatti},

doi = {10.1021/acssensors.3c00073},

issn = {2379-3694},

year  = {2023},

date = {2023-07-01},

journal = {ACS Sens},

volume = {8},

number = {7},

pages = {2533--2542},

abstract = {This manuscript proposes a new dual-mode cell imaging system for studying the relationships between calcium dynamics and the contractility process of cardiomyocytes derived from human-induced pluripotent stem cells. Practically, this dual-mode cell imaging system provides simultaneously both live cell calcium imaging and quantitative phase imaging based on digital holographic microscopy. Specifically, thanks to the development of a robust automated image analysis, simultaneous measurements of both intracellular calcium, a key player of excitation-contraction coupling, and the quantitative phase image-derived dry mass redistribution, reflecting the effective contractility, namely, the contraction and relaxation processes, were achieved. Practically, the relationships between calcium dynamics and the contraction-relaxation kinetics were investigated in particular through the application of two drugs─namely, isoprenaline and E-4031─known to act precisely on calcium dynamics. Specifically, this new dual-mode cell imaging system enabled us to establish that calcium regulation can be divided into two phases, an early phase influencing the occurrence of the relaxation process followed by a late phase, which although not having a significant influence on the relaxation process affects significantly the beat frequency. In combination with cutting-edge technologies allowing the generation of human stem cell-derived cardiomyocytes, this dual-mode cell monitoring approach therefore represents a very promising technique, particularly in the fields of drug discovery and personalized medicine, to identify compounds likely to act more selectively on specific steps that compose the cardiomyocyte contractility.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid37817802,

title = {A robust and reliable methodology to perform GECI-based multi-time point neuronal calcium imaging within mixed cultures of human iPSC-derived cortical neurons},

author = {Niraj Patel and Vincent Ouellet and François Paquet-Mercier and Nizar Chetoui and Erik Bélanger and Marie-Eve Paquet and Antoine G Godin and Pierre Marquet},

doi = {10.3389/fnins.2023.1247397},

issn = {1662-4548},

year  = {2023},

date = {2023-01-01},

journal = {Front Neurosci},

volume = {17},

pages = {1247397},

abstract = {INTRODUCTION: Human induced pluripotent stem cells (iPSCs), with their ability to generate human neural cells (astrocytes and neurons) from patients, hold great promise for understanding the pathophysiology of major neuropsychiatric diseases such as schizophrenia and bipolar disorders, which includes alterations in cerebral development. Indeed, the  neurodifferentiation of iPSCs, while recapitulating certain major stages of neurodevelopment , makes it possible to obtain networks of living human neurons. The culture model presented is particularly attractive within this framework since it involves iPSC-derived neural cells, which more specifically differentiate into cortical neurons of diverse types (in particular glutamatergic and GABAergic) and astrocytes. However, these  neuronal networks, which may be heterogeneous in their degree of differentiation, remain challenging to bring to an appropriate level of maturation. It is therefore necessary to develop tools capable of analyzing a large number of cells to assess this maturation process. Calcium (Ca) imaging, which has been extensively developed, undoubtedly offers an incredibly good approach, particularly in its versions using genetically encoded calcium indicators. However, in the context of these iPSC-derived neural cell cultures, there is a lack of studies that propose Ca imaging methods that can finely characterize the evolution of neuronal maturation during the neurodifferentiation process.nnMETHODS: In this study, we propose a robust and reliable method for specifically measuring neuronal activity at two different time points of the neurodifferentiation process in such human neural cultures. To this end, we have developed a specific Ca signal analysis procedure and tested a series of different AAV serotypes to obtain expression levels of GCaMP6f under the control of the neuron-specific human synapsin1 (hSyn) promoter.nnRESULTS: The retro serotype has been found to be the most efficient in driving the expression of the GCaMP6f and is compatible with multi-time point neuronal Ca imaging in our human iPSC-derived neural cultures. An AAV2/retro carrying GCaMP6f under the hSyn promoter (AAV2/retro-hSyn-GCaMP6f) is an efficient vector that we have identified. To establish the method, calcium measurements were carried out at two time points in the neurodifferentiation process with both hSyn and CAG promoters, the latter being known to provide high transient gene expression across various cell types.nnDISCUSSION: Our results stress that this methodology involving AAV2/retro-hSyn-GCaMP6f is suitable for specifically measuring neuronal calcium activities over multiple time points and is compatible with the neurodifferentiation process in our mixed human neural cultures.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid36528859,

title = {Psychotic features, particularly mood incongruence, as a hallmark of severity of bipolar I disorder},

author = {Julien Elowe and Julie Vallat and Enrique Castelao and Marie-Pierre F Strippoli and Mehdi Gholam and Setareh Ranjbar and Jennifer Glaus and Kathleen Merikangas and Benjamin Lavigne and Pierre Marquet and Martin Preisig and Caroline L Vandeleur},

doi = {10.1186/s40345-022-00280-6},

issn = {2194-7511},

year  = {2022},

date = {2022-12-01},

journal = {Int J Bipolar Disord},

volume = {10},

number = {1},

pages = {31},

abstract = {BACKGROUND: The occurrence of psychotic features within mood episodes in patients with bipolar I disorder (BD I) has been associated in some studies with a more severe clinical and socio-professional profile. In contrast, other studies establishing the associations of psychotic features in BD I, and in particular of mood-congruent (MC) and mood-incongruent (MI) features, with clinical characteristics have yielded contradictory results. However, many pre-existing studies have been affected by serious methodological limitations. Using a sample of thoroughly assessed patients with BD I our aims were to: (1) establish the proportion of those with MI and MC features, and (2) compare BD I patients with and without psychotic features as well as those with MI to those with MC features on a wide array of socio-demographic and clinical characteristics including course, psychiatric comorbidity and treatment.nnMETHODS: A sample of 162 treated patients with BD I (60.5% female, mean age = 41.4 (s.d: 10.2) years) was recruited within a large family study of mood disorders. Clinical, course and treatment characteristics relied on information elicited through direct diagnostic interviews, family history reports and medical records.nnRESULTS: (1) A total of 96 patients (59.3%) had experienced psychotic features over their lifetime. Among them, 44.8% revealed MI features at least once in their lives. (2) Patients with psychotic features were much less likely to be professionally active, revealed alcohol abuse more frequently and used health care, particularly inpatient treatment, more frequently than those without psychotic features. Within patients with psychotic symptoms, those with MI features showed more clinical severity in terms of a higher likelihood of reporting hallucinations, suicidal attempts and comorbid cannabis dependence.nnCONCLUSION: Our data provide additional support for both the distinction between BD-I with and without psychotic features as well as the distinction between MI and MC psychotic features. The more severe course of patients with psychotic features, and particularly those with MI psychotic features, highlights the need for thorough psychopathological evaluations to assess the presence of these symptoms to install appropriate treatment.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid35386056,

title = {Environmental factors in offspring of parents with mood disorders and their role in parent-child transmission: findings from a 14-year prospective high-risk study},

author = {Flore Moulin and Mehdi Gholam and Marie-Pierre F Strippoli and Enrique Castelao and Kathleen R Merikangas and Emma K Stapp and Pierre Marquet and Jean-Michel Aubry and Kerstin J Plessen and Francesca Di Giacomo and Jennifer Glaus and Giorgio Pistis and Benjamin Lavigne and Julien Elowe and Setareh Ranjbar and Martin Preisig and Caroline L Vandeleur},

doi = {10.1186/s40345-022-00257-5},

issn = {2194-7511},

year  = {2022},

date = {2022-04-01},

journal = {Int J Bipolar Disord},

volume = {10},

number = {1},

pages = {11},

abstract = {BACKGROUND: The factors involved in the transmission of mood disorders are only partially elucidated. Aside from genes, the family environment might play a crucial role in parent-child transmission. Our goals were to (1) assess the associations of parental bipolar disorder (BPD) and Major Depressive Disorder (MDD) with individual or shared family environmental factors, including traumatic events in offspring, parental separation, family cohesion and parental attitudes; and 2) test whether these factors were mediators of the association between exposure to parental mood disorders and the onset of these disorders in offspring.nnMETHODS: The sample stems from an ongoing family high-risk study of mood disorders conducted in the French-speaking part of Switzerland. Given the strong impact of the age of onset of parental disorders on their transmission to children, parental disorders were dichotomized according to the onset (cut-off 21 years). Probands with early-onset (n = 30) and later-onset BPD (n = 51), early-onset (n = 21) and later-onset MDD (n = 47) and controls (n = 65), along with their spouses (n = 193) and offspring (n = 388; < 18 years on study inclusion), were assessed over a mean follow-up duration of 14 years (s.d: 4.6). The environmental measures were based on reports by offspring collected before the onset of their first mood episode.nnRESULTS: Offspring of probands with later-onset BPD and offspring of probands with both early-onset and later-onset MDD reported traumatic events more frequently than comparison offspring, whereas exposure to parental separation was more frequent in all groups of high-risk offspring. Moreover, several familial environment scores including parenting attitudes differed between offspring of probands with BPD and comparison offspring. However, none of these factors were mediators of the parent-child transmission of BPD. Among the environmental factors, traumatic events were shown to be modest mediators of the transmission of early-onset MDD.nnCONCLUSIONS: Our data do not support the implication of the assessed environmental factors in the parent-child transmission of BPD. In contrast to BPD, traumatic events partially mediate the parent-child transmission of early-onset MDD, which has important implications for intervention and prevention. Early therapeutic efforts in offspring exposed to these events are likely to reduce their deleterious impact on the risk of subsequent MDD.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}