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2601 Chemin de la Canardière
Québec (Québec)
G1J 2G3 Canada
Professeur-adjoint sous-octroi, Département de psychiatrie et neurosciences, Université Laval
Axe de recherche:
Neurosciences intégratives et thérapies expérimentales
Comment acquérons-nous de nouvelles compétences? Comment optimisons-nous les tâches précédemment acquises?
Notre laboratoire de neurosciences des systèmes étudie les processus neuronaux menant à l’apprentissage et l’attention. Nous sommes intéressés plus particulièrement au rôle des catécholamines – la noradrénaline et la dopamine – dans le comportement nécessitant l’apprentissage. Nos objectifs principaux sont de déterminer quand les neurones dopaminergiques ou noradrénergiques sont actifs, quelles régions du cerveau causent cette activité et comment le relâchement de dopamine ou noradrénaline affecte l’activité neuronale dans les régions cibles. Ultimement, nous désirons utiliser ces connaissances de bases sur la fonction des catécholamines dans le comportement pour mieux comprendre le rôle des catécholamines dans les désordres neuropsychiatriques. Nous combinons l’optogénétiques, la microscopie à deux photons, l’électrophysiologie et les approches computationnelles pour définir l’encodage neuronal durant le comportement.
Si vous êtes intéressés à joindre le laboratoire, envoyez-nous un email.
J’ai précédemment fait mes études postdoctorales dans le laboratoire du Dr. Mriganka Sur au Massachusetts Institute of Technology, dans lequel j’ai étudié les circuits contrôlant la noradrénaline et leur implication dans l’éveil, l’exécution de tâches ainsi que dans l’apprentissage par renforcement. Avant de joindre le laboratoire de Dr. Sur, j’ai étudié la neurogénèse adulte dans le laboratoire du Dr. Armen Saghatelyan à l’Université Laval. J’ai obtenu mon baccalauréat en Génie Physique, avec concentration en optique et photonique.
Michael A Kelberman; Ellen Rodberg; Ehsan Arabzadeh; Chloe J Bair-Marshall; Craig W Berridge; Esther Berrocoso; Vincent Breton-Provencher; Daniel J Chandler; Alicia Che; Oscar Davy; David M Devilbiss; Anthony M Downs; Gabrielle Drummond; Roman Dvorkin; Zeinab Fazlali; Robert C Froemke; Erin Glennon; Joshua I Gold; Hiroki Ito; Xiaolong Jiang; Joshua P Johansen; Alfred P Kaye; Jenny R Kim; Chao-Cheng Kuo; Rong-Jian Liu; Yang Liu; Meritxell Llorca-Torralba; Jordan G McCall; Zoe A McElligott; Andrew M McKinney; Cristina Miguelez; Ming-Yuan Min; Alexandra C Nowlan; Mohsen Omrani; Gina R Poe; Anthony Edward Pickering; Yadollah Ranjbar-Slamloo; Jone Razquin; Charles Rodenkirch; Anna C Sales; Rath Satyasambit; Stephen D Shea; Mriganka Sur; John Arthur Tkaczynski; Sonia Torres-Sanchez; Akira Uematsu; Chayla R Vazquez; Amelien Vreven; Qi Wang; Barry D Waterhouse; Hsiu-Wen Yang; Jen-Hau Yang; Liping Zhao; Ioannis S Zouridis; David Weinshenker; Elena Vazey; Nelson K Totah
Diversity of ancestral brainstem noradrenergic neurons across species and multiple biological factors Article de journal
Dans: bioRxiv, 2024, ISSN: 2692-8205.
@article{pmid39464004,
title = {Diversity of ancestral brainstem noradrenergic neurons across species and multiple biological factors},
author = {Michael A Kelberman and Ellen Rodberg and Ehsan Arabzadeh and Chloe J Bair-Marshall and Craig W Berridge and Esther Berrocoso and Vincent Breton-Provencher and Daniel J Chandler and Alicia Che and Oscar Davy and David M Devilbiss and Anthony M Downs and Gabrielle Drummond and Roman Dvorkin and Zeinab Fazlali and Robert C Froemke and Erin Glennon and Joshua I Gold and Hiroki Ito and Xiaolong Jiang and Joshua P Johansen and Alfred P Kaye and Jenny R Kim and Chao-Cheng Kuo and Rong-Jian Liu and Yang Liu and Meritxell Llorca-Torralba and Jordan G McCall and Zoe A McElligott and Andrew M McKinney and Cristina Miguelez and Ming-Yuan Min and Alexandra C Nowlan and Mohsen Omrani and Gina R Poe and Anthony Edward Pickering and Yadollah Ranjbar-Slamloo and Jone Razquin and Charles Rodenkirch and Anna C Sales and Rath Satyasambit and Stephen D Shea and Mriganka Sur and John Arthur Tkaczynski and Sonia Torres-Sanchez and Akira Uematsu and Chayla R Vazquez and Amelien Vreven and Qi Wang and Barry D Waterhouse and Hsiu-Wen Yang and Jen-Hau Yang and Liping Zhao and Ioannis S Zouridis and David Weinshenker and Elena Vazey and Nelson K Totah},
doi = {10.1101/2024.10.14.618224},
issn = {2692-8205},
year = {2024},
date = {2024-10-01},
journal = {bioRxiv},
abstract = {The brainstem region, locus coeruleus (LC), has been remarkably conserved across vertebrates. Evolution has woven the LC into wide-ranging neural circuits that influence functions as broad as autonomic systems, the stress response, nociception, sleep, and high-level cognition among others. Given this conservation, there is a strong possibility that LC activity is inherently similar across species, and furthermore that age, sex, and brain state influence LC activity similarly across species. The degree to which LC activity is homogenous across these factors, however, has never been assessed due to the small sample size of individual studies. Here, we pool data from 20 laboratories (1,855 neurons) and show diversity across both intrinsic and extrinsic factors such as species, age, sex and brain state. We use a negative binomial regression model to compare activity from male monkeys, and rats and mice of both sexes that were recorded across brain states from brain slices or under different anesthetics or during wakefulness . LC activity differed due to complex interactions of species, sex, and brain state. The LC became more active during aging, independent of sex. Finally, in contrast to the foundational principle that all species express two distinct LC firing modes ("tonic" or "phasic"), we discovered great diversity within spontaneous LC firing patterns. Different factors were associated with higher incidence of some firing modes. We conclude that the activity of the evolutionarily-ancient LC is not conserved. Inherent differences due to age and species-sex-brain state interactions have implications for understanding the role of LC in species-specific naturalistic behavior, as well as in psychiatric disorders, cardiovascular disease, immunology, and metabolic disorders.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Gabrielle T Drummond; Arundhati Natesan; Marco Celotto; Jennifer Shih; Prachi Ojha; Yuma Osako; Jiho Park; Grayson O Sipe; Kyle R Jenks; Vincent Breton-Provencher; Paul C Simpson; Stefano Panzeri; Mriganka Sur
Cortical norepinephrine-astrocyte signaling critically mediates learned behavior Article de journal
Dans: bioRxiv, 2024, ISSN: 2692-8205.
@article{pmid39484425,
title = {Cortical norepinephrine-astrocyte signaling critically mediates learned behavior},
author = {Gabrielle T Drummond and Arundhati Natesan and Marco Celotto and Jennifer Shih and Prachi Ojha and Yuma Osako and Jiho Park and Grayson O Sipe and Kyle R Jenks and Vincent Breton-Provencher and Paul C Simpson and Stefano Panzeri and Mriganka Sur},
doi = {10.1101/2024.10.24.620009},
issn = {2692-8205},
year = {2024},
date = {2024-10-01},
journal = {bioRxiv},
abstract = {Updating behavior based on feedback from the environment is a crucial means by which organisms learn and develop optimal behavioral strategies. Norepinephrine (NE) release from the locus coeruleus (LC) has been shown to mediate learned behaviors such that in a task with graded stimulus uncertainty and performance, a high level of NE released after an unexpected outcome causes improvement in subsequent behavior. Yet, how the transient activity of LC-NE neurons, lasting tens of milliseconds, influences behavior several seconds later, is unclear. Here, we show that NE acts directly on cortical astrocytes via Adra1a adrenergic receptors to elicit sustained increases in intracellular calcium. Chemogenetic blockade of astrocytic calcium elevation prevents the improvement in behavioral performance. NE-activated calcium invokes purinergic pathways in cortical astrocytes that signal to neurons; pathway-specific astrocyte gene expression is altered in mice trained on the task, and blocking neuronal adenosine-sensitive A1 receptors also prevents post-reinforcement behavioral gain. Finally, blocking either astrocyte calcium dynamics or A1 receptors alters encoding of the task in prefrontal cortex neurons, preventing the post-reinforcement change in discriminability of rewarded and unrewarded stimuli underlying behavioral improvement. Together, these data demonstrate that astrocytes, rather than indirectly reflecting neuronal drive, play a direct, instrumental role in representing task-relevant information and signaling to neurons to mediate a fundamental component of learning in the brain.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Nithik Chintalacheruvu; Anagha Kalelkar; Jöel Boutin; Vincent Breton-Provencher; Rafiq Huda
A cortical locus for modulation of arousal states Article de journal
Dans: bioRxiv, 2024, ISSN: 2692-8205.
@article{pmid38826269,
title = {A cortical locus for modulation of arousal states},
author = {Nithik Chintalacheruvu and Anagha Kalelkar and Jöel Boutin and Vincent Breton-Provencher and Rafiq Huda},
doi = {10.1101/2024.05.24.595859},
issn = {2692-8205},
year = {2024},
date = {2024-05-01},
journal = {bioRxiv},
abstract = {Fluctuations in global arousal are key determinants of spontaneous cortical activity and function. Several subcortical structures, including neuromodulator nuclei like the locus coeruleus (LC), are involved in the regulation of arousal. However, much less is known about the role of cortical circuits that provide top-down inputs to arousal-related subcortical structures. Here, we investigated the role of a major subdivision of the prefrontal cortex, the anterior cingulate cortex (ACC), in arousal modulation. Pupil size, facial movements, heart rate, and locomotion were used as non-invasive measures of arousal and behavioral state. We designed a closed loop optogenetic system based on machine vision and found that real time inhibition of ACC activity during pupil dilations suppresses ongoing arousal events. In contrast, inhibiting activity in a control cortical region had no effect on arousal. Fiber photometry recordings showed that ACC activity scales with the magnitude of spontaneously occurring pupil dilations/face movements independently of locomotion. Moreover, optogenetic ACC activation increases arousal independently of locomotion. In addition to modulating global arousal, ACC responses to salient sensory stimuli scaled with the size of evoked pupil dilations. Consistent with a role in sustaining saliency-linked arousal events, pupil responses to sensory stimuli were suppressed with ACC inactivation. Finally, our results comparing arousal-related ACC and norepinephrinergic LC neuron activity support a role for the LC in initiation of arousal events which are modulated in real time by the ACC. Collectively, our experiments identify the ACC as a key cortical site for sustaining momentary increases in arousal and provide the foundation for understanding cortical-subcortical dynamics underlying the modulation of arousal states.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Emmeraude Tanguay; Sarah-Julie Bouchard; Martin Lévesque; Paul De Koninck; Vincent Breton-Provencher
Shining light on the noradrenergic system Article de journal
Dans: Neurophotonics, vol. 10, no 4, p. 044406, 2023, ISSN: 2329-423X.
@article{pmid37766924,
title = {Shining light on the noradrenergic system},
author = {Emmeraude Tanguay and Sarah-Julie Bouchard and Martin Lévesque and Paul De Koninck and Vincent Breton-Provencher},
doi = {10.1117/1.NPh.10.4.044406},
issn = {2329-423X},
year = {2023},
date = {2023-10-01},
journal = {Neurophotonics},
volume = {10},
number = {4},
pages = {044406},
abstract = {Despite decades of research on the noradrenergic system, our understanding of its impact on brain function and behavior remains incomplete. Traditional recording techniques are challenging to implement for investigating noradrenergic activity, due to the relatively small size and the position in the brain of the locus coeruleus (LC), the primary location for noradrenergic neurons. However, recent advances in optical and fluorescent methods have enabled researchers to study the LC more effectively. Use of genetically encoded calcium indicators to image the activity of noradrenergic neurons and biosensors that monitor noradrenaline release with fluorescence can be an indispensable tool for studying noradrenergic activity. In this review, we examine how these methods are being applied to record the noradrenergic system in the rodent brain during behavior.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Yarden Cohen; Tatiana A Engel; Christopher Langdon; Grace W Lindsay; Torben Ott; Megan A K Peters; James M Shine; Vincent Breton-Provencher; Srikanth Ramaswamy
Recent Advances at the Interface of Neuroscience and Artificial Neural Networks Article de journal
Dans: J Neurosci, vol. 42, no 45, p. 8514–8523, 2022, ISSN: 1529-2401.
@article{pmid36351830,
title = {Recent Advances at the Interface of Neuroscience and Artificial Neural Networks},
author = {Yarden Cohen and Tatiana A Engel and Christopher Langdon and Grace W Lindsay and Torben Ott and Megan A K Peters and James M Shine and Vincent Breton-Provencher and Srikanth Ramaswamy},
doi = {10.1523/JNEUROSCI.1503-22.2022},
issn = {1529-2401},
year = {2022},
date = {2022-11-01},
journal = {J Neurosci},
volume = {42},
number = {45},
pages = {8514--8523},
abstract = {Biological neural networks adapt and learn in diverse behavioral contexts. Artificial neural networks (ANNs) have exploited biological properties to solve complex problems. However, despite their effectiveness for specific tasks, ANNs are yet to realize the flexibility and adaptability of biological cognition. This review highlights recent advances in computational and experimental research to advance our understanding of biological and artificial intelligence. In particular, we discuss critical mechanisms from the cellular, systems, and cognitive neuroscience fields that have contributed to refining the architecture and training algorithms of ANNs. Additionally, we discuss how recent work used ANNs to understand complex neuronal correlates of cognition and to process high throughput behavioral data.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Vincent Breton-Provencher; Gabrielle T Drummond; Jiesi Feng; Yulong Li; Mriganka Sur
Spatiotemporal dynamics of noradrenaline during learned behaviour Article de journal
Dans: Nature, vol. 606, no 7915, p. 732–738, 2022, ISSN: 1476-4687.
@article{pmid35650441,
title = {Spatiotemporal dynamics of noradrenaline during learned behaviour},
author = {Vincent Breton-Provencher and Gabrielle T Drummond and Jiesi Feng and Yulong Li and Mriganka Sur},
doi = {10.1038/s41586-022-04782-2},
issn = {1476-4687},
year = {2022},
date = {2022-06-01},
journal = {Nature},
volume = {606},
number = {7915},
pages = {732--738},
abstract = {Noradrenaline released from the locus coeruleus (LC) is a ubiquitous neuromodulator that has been linked to multiple functions including arousal, action and sensory gain, and learning. Whether and how activation of noradrenaline-expressing neurons in the LC (LC-NA) facilitates different components of specific behaviours is unknown. Here we show that LC-NA activity displays distinct spatiotemporal dynamics to enable two functions during learned behaviour: facilitating task execution and encoding reinforcement to improve performance accuracy. To examine these functions, we used a behavioural task in mice with graded auditory stimulus detection and task performance. Optogenetic inactivation of the LC demonstrated that LC-NA activity was causal for both task execution and optimization. Targeted recordings of LC-NA neurons using photo-tagging, two-photon micro-endoscopy and two-photon output monitoring showed that transient LC-NA activation preceded behavioural execution and followed reinforcement. These two components of phasic activity were heterogeneously represented in LC-NA cortical outputs, such that the behavioural response signal was higher in the motor cortex and facilitated task execution, whereas the negative reinforcement signal was widely distributed among cortical regions and improved response sensitivity on the subsequent trial. Modular targeting of LC outputs thus enables diverse functions, whereby some noradrenaline signals are segregated among targets, whereas others are broadly distributed.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Rajeev V Rikhye; Murat Yildirim; Ming Hu; Vincent Breton-Provencher; Mriganka Sur
Reliable Sensory Processing in Mouse Visual Cortex through Cooperative Interactions between Somatostatin and Parvalbumin Interneurons Article de journal
Dans: J Neurosci, vol. 41, no 42, p. 8761–8778, 2021, ISSN: 1529-2401.
@article{pmid34493543,
title = {Reliable Sensory Processing in Mouse Visual Cortex through Cooperative Interactions between Somatostatin and Parvalbumin Interneurons},
author = {Rajeev V Rikhye and Murat Yildirim and Ming Hu and Vincent Breton-Provencher and Mriganka Sur},
doi = {10.1523/JNEUROSCI.3176-20.2021},
issn = {1529-2401},
year = {2021},
date = {2021-10-01},
journal = {J Neurosci},
volume = {41},
number = {42},
pages = {8761--8778},
abstract = {Intrinsic neuronal variability significantly limits information encoding in the primary visual cortex (V1). However, under certain conditions, neurons can respond reliably with highly precise responses to the same visual stimuli from trial to trial. This suggests that there exists intrinsic neural circuit mechanisms that dynamically modulate the intertrial variability of visual cortical neurons. Here, we sought to elucidate the role of different inhibitory interneurons (INs) in reliable coding in mouse V1. To study the interactions between somatostatin-expressing interneurons (SST-INs) and parvalbumin-expressing interneurons (PV-INs), we used a dual-color calcium imaging technique that allowed us to simultaneously monitor these two neural ensembles while awake mice, of both sexes, passively viewed natural movies. SST neurons were more active during epochs of reliable pyramidal neuron firing, whereas PV neurons were more active during epochs of unreliable firing. SST neuron activity lagged that of PV neurons, consistent with a feedback inhibitory SST→PV circuit. To dissect the role of this circuit in pyramidal neuron activity, we used temporally limited optogenetic activation and inactivation of SST and PV interneurons during periods of reliable and unreliable pyramidal cell firing. Transient firing of SST neurons increased pyramidal neuron reliability by actively suppressing PV neurons, a proposal that was supported by a rate-based model of V1 neurons. These results identify a cooperative functional role for the SST→PV circuit in modulating the reliability of pyramidal neuron activity. Cortical neurons often respond to identical sensory stimuli with large variability. However, under certain conditions, the same neurons can also respond highly reliably. The circuit mechanisms that contribute to this modulation remain unknown. Here, we used novel dual-wavelength calcium imaging and temporally selective optical perturbation to identify an inhibitory neural circuit in visual cortex that can modulate the reliability of pyramidal neurons to naturalistic visual stimuli. Our results, supported by computational models, suggest that somatostatin interneurons increase pyramidal neuron reliability by suppressing parvalbumin interneurons via the inhibitory SST→PV circuit. These findings reveal a novel role of the SST→PV circuit in modulating the fidelity of neural coding critical for visual perception.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Vincent Breton-Provencher; Gabrielle T Drummond; Mriganka Sur
Locus Coeruleus Norepinephrine in Learned Behavior: Anatomical Modularity and Spatiotemporal Integration in Targets Article de journal
Dans: Front Neural Circuits, vol. 15, p. 638007, 2021, ISSN: 1662-5110.
@article{pmid34163331,
title = {Locus Coeruleus Norepinephrine in Learned Behavior: Anatomical Modularity and Spatiotemporal Integration in Targets},
author = {Vincent Breton-Provencher and Gabrielle T Drummond and Mriganka Sur},
doi = {10.3389/fncir.2021.638007},
issn = {1662-5110},
year = {2021},
date = {2021-01-01},
journal = {Front Neural Circuits},
volume = {15},
pages = {638007},
abstract = {The locus coeruleus (LC), a small brainstem nucleus, is the primary source of the neuromodulator norepinephrine (NE) in the brain. The LC receives input from widespread brain regions, and projects throughout the forebrain, brainstem, cerebellum, and spinal cord. LC neurons release NE to control arousal, but also in the context of a variety of sensory-motor and behavioral functions. Despite its brain-wide effects, much about the role of LC-NE in behavior and the circuits controlling LC activity is unknown. New evidence suggests that the modular input-output organization of the LC could enable transient, task-specific modulation of distinct brain regions. Future work must further assess whether this spatial modularity coincides with functional differences in LC-NE subpopulations acting at specific times, and how such spatiotemporal specificity might influence learned behaviors. Here, we summarize the state of the field and present new ideas on the role of LC-NE in learned behaviors.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Rafiq Huda; Grayson O Sipe; Vincent Breton-Provencher; K Guadalupe Cruz; Gerald N Pho; Elie Adam; Liadan M Gunter; Austin Sullins; Ian R Wickersham; Mriganka Sur
Distinct prefrontal top-down circuits differentially modulate sensorimotor behavior Article de journal
Dans: Nat Commun, vol. 11, no 1, p. 6007, 2020, ISSN: 2041-1723.
@article{pmid33243980,
title = {Distinct prefrontal top-down circuits differentially modulate sensorimotor behavior},
author = {Rafiq Huda and Grayson O Sipe and Vincent Breton-Provencher and K Guadalupe Cruz and Gerald N Pho and Elie Adam and Liadan M Gunter and Austin Sullins and Ian R Wickersham and Mriganka Sur},
doi = {10.1038/s41467-020-19772-z},
issn = {2041-1723},
year = {2020},
date = {2020-11-01},
journal = {Nat Commun},
volume = {11},
number = {1},
pages = {6007},
abstract = {Sensorimotor behaviors require processing of behaviorally relevant sensory cues and the ability to select appropriate responses from a vast behavioral repertoire. Modulation by the prefrontal cortex (PFC) is thought to be key for both processes, but the precise role of specific circuits remains unclear. We examined the sensorimotor function of anatomically distinct outputs from a subdivision of the mouse PFC, the anterior cingulate cortex (ACC). Using a visually guided two-choice behavioral paradigm with multiple cue-response mappings, we dissociated the sensory and motor response components of sensorimotor control. Projection-specific two-photon calcium imaging and optogenetic manipulations show that ACC outputs to the superior colliculus, a key midbrain structure for response selection, principally coordinate specific motor responses. Importantly, ACC outputs exert control by reducing the innate response bias of the superior colliculus. In contrast, ACC outputs to the visual cortex facilitate sensory processing of visual cues. Our results ascribe motor and sensory roles to ACC projections to the superior colliculus and the visual cortex and demonstrate for the first time a circuit motif for PFC function wherein anatomically non-overlapping output pathways coordinate complementary but distinct aspects of visual sensorimotor behavior.},
keywords = {},
pubstate = {published},
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}
Amanda J Whipple; Vincent Breton-Provencher; Hannah N Jacobs; Udbhav K Chitta; Mriganka Sur; Phillip A Sharp
Imprinted Maternally Expressed microRNAs Antagonize Paternally Driven Gene Programs in Neurons Article de journal
Dans: Mol Cell, vol. 78, no 1, p. 85–95.e8, 2020, ISSN: 1097-4164.
@article{pmid32032531,
title = {Imprinted Maternally Expressed microRNAs Antagonize Paternally Driven Gene Programs in Neurons},
author = {Amanda J Whipple and Vincent Breton-Provencher and Hannah N Jacobs and Udbhav K Chitta and Mriganka Sur and Phillip A Sharp},
doi = {10.1016/j.molcel.2020.01.020},
issn = {1097-4164},
year = {2020},
date = {2020-04-01},
journal = {Mol Cell},
volume = {78},
number = {1},
pages = {85--95.e8},
abstract = {Imprinted genes with parental-biased allelic expression are frequently co-regulated and enriched in common biological pathways. Here, we functionally characterize a large cluster of microRNAs (miRNAs) expressed from the maternally inherited allele ("maternally expressed") to explore the molecular and cellular consequences of imprinted miRNA activity. Using an induced neuron (iN) culture system, we show that maternally expressed miRNAs from the miR-379/410 cluster direct the RNA-induced silencing complex (RISC) to transcriptional and developmental regulators, including paternally expressed transcripts like Plagl1. Maternal deletion of this imprinted miRNA cluster resulted in increased protein levels of several targets and upregulation of a broader transcriptional program regulating synaptic transmission and neuronal function. A subset of the transcriptional changes resulting from miR-379/410 deletion can be attributed to de-repression of Plagl1. These data suggest maternally expressed miRNAs antagonize paternally driven gene programs in neurons.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
